We have developed a model system that enables us to perform unique studies that directly address this problem. We investigate the neural basis for respiration in the isolated neonate rat brainstem, a preparation that offers simultaneous access to cellular, synaptic and network properties. In this preparation, the circuitry mediating respiration has been localized in a discrete region of brainstem, which can be isolated in a 300 m m thick slice. Using the slice, sophisticated techniques are used to characterize the cellular and synaptic properties of neurons involved in respiration, while at the same time characterizing their interactions at the network level, and recording motor output. Exploiting this preparation, we intend to understand how homeostatically regulated breathing movements occur, and in course, principles of integrative organization in the nervous system.

We use whole-cell patch-clamp recording techniques to determine cellular and synaptic properties, including the pharmacology of identified synaptic interactions. We are presently testing our hypothesis that pacemaker neurons underlie the generation of respiratory rhythm. We have several projects looking at general mechanisms underlying the control of neuronal excitability (in functionally identified cells). In particular, we are interested in the interaction among amino acid, amine and peptide neurotransmitters. We have initiated a major program in molecular neurobiology to determine the interesting and unique molecules associated with identified cells in this network; presently, we are developing RT-PCR and mRNA differential display for this purpose. We are also interested in modeling, for which we have excellent computer facilities.

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neurobiology, neuroscience, Neural mechanisms underlying movement and homeostasis, breathing