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UCLA Department of Physiology & Cardiovascular Research Lab
Kenneth P. Roos, Ph.D., Director
Maria C. Jordan, M.D., Surgeon/Sonographer
Who We Are:
The Mouse Physiology Laboratory is a core research facility dedicated to the phenotypic assessment of mouse and rat models of disease using a wide variety of integrative approaches. Our aim is to provide a cost effective means for investigators to reveal important functional aspects of their mouse and rodent models without having to individually invest in training personnel in specialized procedures and purchase expensive instrumentation such as ultrasound machines and telemetry equipment. We work with you to design and implement the study along with any associated grant and manuscript preparation. The laboratory has collaborated with dozens of investigators at UCLA and other institutions worldwide on a wide range of studies leading to numerous peer reviewed publications.
What We Do:
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Echocardiography
: 2-D guided M-Mode & Doppler with 15 MHz Siemens Acuson Sequoia provides high resolution image analysis of chamber dimensions, wall thicknesses and ventricular function in lightly sedated mice or rats. Dobutamine stress-echo also available.
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on-invasive Tail Cuff Blood Pressures
: For high throughput screening of restrained awake mice or rats.
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Hemodynamics
: Carotid or Femoral catheterizations for arterial and venous pressures, ECGs, cardiac output via Doppler flow probes and left ventricular pressures via Millar Catheter. Calculated heart rates, CO & LV dP/dT. Inotropic agent (Dobutamine/ Isoproterenol challenge) and baroreceptor sensitivity stress testing. In anesthetized mice or rats, non-survival.
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urgical Procedures
: Trans-aortic constriction (TAC), myocardial infarctions via coronary artery occlusions, stem cell therapy, salt loading via DOCA pellets or diet, nephrectomy, and osmotic pump implantation. Survival surgery; mice and rats.
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Remote Telemetry
: Implantation of arterial blood pressure transducers or ECG/temperature/activity transducers. 24/7 recording of BP, ECG, core body temperature and activity from awake, circadian rhythm analysis in free-roaming mice and rats.
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Autonomic Nervous System Testing
: Blood pressure and heart rate regulation. Heart rate variability power spectral analysis.
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Exercise Testing
: Exercise stress and endurance testing. If combined with telemetry, pressures & ECG can be recorded during exercise. (Mice only)
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Metabolic Testing
: Measurement of oxygen consumption and CO2 generation via indirect calorimetry to obtain values of resting metabolic rate. When combined with exercise and telemetry, the system can provide measurements of heart rate, temperature and VO2max at rest and during high-intensity exercise. (Mice only).
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Postmortem Morphometry
: Organ dissection for measurement (mass, size) or fixation for histology.(Rats and Mice)
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Langendorff Perfused Cell Isolation and Function
: Isolation of adult ventricular myocytes via Langendorff retrograde perfusion. Functional evaluation of isolated cell contractility in mice or rats.
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Drug Testing:
Certification testing of new drugs or substances intended for human use by performing FDA approved 21 CRF GLP studies on rodent models.
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Custom Designed Phenotypic Assessment
: We will work with you to custom design the best possible experiments to reveal the phenotype of your animal model.
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Consulting
: We will consult with any investigator on phenotypic experimental design and data analysis.
How to Contact Us:
E-mail Dr. Roos: kroos@mednet.ucla.edu
Visit our website for more information: http://www.physiology.ucla.edu/mouselab
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