• Provide instrumentation, as well as technical and professional assistance, for performing laser-based flow cytometry.
• Multi-color leukocyte subset enumeration, quantitation of cell surface antigen expression, measurement of DNA content, measurement of intracellular antigens (alone or combined with DNA content analysis and/or cell surface immunofluorescence), calcium flux evaluation (alone or combined with cell surface immunofluorescence), apoptosis measurements (alone or combined with DNA content analysis or cell surface immunofluorescence), measurement of DNA-RNA content and assessment of fluorescent protein expression. Cell sorting options include aseptic sorting, sorting of potentially biohazardous specimens and single cell cloning.
• Consistent and extensive instrumentation quality control.
• Consultation with the manager, as well as continuous availability of expert advice on flow cytometry during data collection and analysis, through personnel in the laboratory.
• Education through formal courses is provided as needed to users, so they may learn basic flow cytometry principles and learn to run their own specimens on the analytic flow cytometers under supervision of the shared resource staff. Classes on nucleic acid flow cytometry are also offered.

Costs:
There is a tiered fee structure. Costs for JCCC members are listed below; for non-members, please refer to our website for details.

Description	Fees
Sorting	$82/hr
Calcium flux measurements	$82/hr
Acquisition and analysis by FCSR staff (sample stained by researcher)	$82/hr
Acquisition and analysis by researcher, with supervision by FCSR staff	$57/hr
Sample stained with antibody provided by FCSR laboratory and data acquired and analyzed by FCSR staff (2 tube minimum)	$20/tube
Reagent purchase by researcher from FCSR	$4 - $8 /aliquot
Data storage devices	$35 - $60
Flow cytometry class	$272
Nucleic acid flow cytometry instructional session	$136
Off-line computer analysis by researcher	$34/hr
Use of the RoboSep cell separator	$30/ 45 mins

Operating Policies:
Faculty investigators, research fellows or others who wish to start using the Flow Cytometry Shared Resource must contact the manager for discussion of their experiments, help with study design and assistance with the procedures and product descriptions of reagents they will need to order. Pilot studies are performed by the lab manager, if necessary, and the study design is perfected. The project is then assigned to one of the flow cytometry technicians. The lab manager remains actively involved in the more complex projects and oversees the technical quality of all work produced by the laboratory. After the initial discussion with the manager, cell sorting experiments are scheduled through the sorter operator. With the exception of developmental work or pilot experiments, the principal investigators are recharged for all time and/or work performed. Details are available on our website at http://cyto.mednet.ucla.edu .